Monday, January 27, 2020

Casablanca Social And Political Mores Of Society Film Studies Essay

Casablanca Social And Political Mores Of Society Film Studies Essay The classical film Casablanca (1942) is a romantic story set in the times of World War II and is about two men who were in love with the same woman. Rick Blaine (Humphrey Bogart) owns the most popular nightclub and gambling hideout Ricks Cafà © Americian that is located in Casablanca. During the war many people wanted to escape Europe for America therefore, people who wanted to escape went to Ricks Cafà ©. Ricks cafà © became heaven for the people who wanted to escape looking to buy illegal letters of transit to allow them to escape. Unexpectedly a gorgeous lady, Ilsa Lazlo (Ingrid Bergman) enters the nightclub with her husband Victor Lazlo (Paul Henreid). Ilsa was Ricks true love who had left him when the Nazis conquered Paris. Ilsa wanted her husband to escape to America; however, she wanted to stay behind in Casablanca after renewing her love with Rick. Though, Casablanca did not end in an entirely happy ending because Ilsa has escaped with Victor. The director (Michael Curtiz) uses many elements throughout the film such as: using actors, dialogue, elements of lighting, elements of camera movements, sound, editing and production design to show the audience the understanding of the whole story. The director used different styles throughout the whole film to send an emotional, and political messages implied by the characters action. Actors The director (Michael Curtiz) uses famous stars Rick (Humphrey Bogart) and Ilsa (Ingrid Bergman) in the romance film Casablanca. Rick as a mysterious handsome cafà © owner with a love past, set up in the business with his friend Sam (Dooley Wilson) as a piano player. In addition, all the refugees are under Ricks protection which means that he sells illegal documents for the refugees who wants o escape to America. Curtiz makes Ricks cafà © seem very real. In addition, the film focuses on Rick clash between love and virtue: since he must choose between doing the right thing by helping Ilsas husband (Victor Laszlo) escape or his love to her. Also Curtiz chooses Ilsa (Ingrid Bergman) to be a beautiful lady who Rick falls in love with, and married to Victor Laszlo (Paul Henreid). Ilsas role is basically a lover and a help a great man (Victor). The movie is a metaphor for American involvement in World War II. Curtiz makes the dialogue very touching and ironically funny. Movement Casablanca obviously prefers closed formal compositions as opposed to open one, and opening framings play a famous role in the visual design and staging of shots (visual design refers to the static organization within shots while visual staging refers to organizational shifts within shots). I will speak about the scene where Rick had his Paris flashback and waiting for Ilsa to show up after she arrived to Casablanca. The shot demonstrated to the audience how the camera was moving from right to left movements from Ricks face to his hand which tips the glass. Moreover, the second shot shows the audience the camera movement that was following Sam the piano player walking on the right towards Rick leaving space between both Sam and Rick and the door was showing in the background. This shot sets up the audience for the approaching scene where Ilsa will enter unexpectedly from the door in the background. After that, Ricks grabs the alcohol bottle so he can pour a drink for himself; by doin g that it showed a clear view of Ilsas entrance. This scene provides attention towards Ilsas entrance. When Ilsa is about to enter the cafe both Rick and Sam immediately turned their heads towards the door, the lights where darkened before Ilsas entrance; and when Ilsa does enter, the lighting of the room is back and it highlights that Ilsa is framed by background door. In this scene there were many pauses on some parts to show the importance of the character or information such as the faces, hands, body movements and eyes. Therefore, by doing the pauses they will grab the attention of the audience towards the specific shot. According to Louis Giannetti, movements that are from right to left physical movement in this direction seems natural however, movements from left to right seems mysteriously tense and uncomfortable. (Giannetti, 2010). Sound As well, the major musical pattern which is the song that Ilsa asked Sam the piano player to play her and Ricks song As Times Goes By. The song showed the audience the love and romance between Ilsa and Rick. The music appeared again when Rick was lost in his sad memories, in the flash back sequence in Paris. By playing the song it will bring to the audience an emotional atmosphere. According Louis Giannetti in Understanding Movies, music merged with lyrics, music acquires a more real content because words have specific references to the film. With or without lyrics, music can be more specific when contrast with film images. (Giannetti, 2010). In Casablanca, while playing the music in Ricks flashback it showed the audience the love between both Rick and Ilsa. Furthermore, according to Louis Giannetti, loud sounds tend to be threatening and intense; however, quiet sounds strike as delicate, hesitant, and often weak. (Giannetti, 2010). Moreover, when Rick remembers the time he spent in Paris with Ilsa, when they were in the car driving or drinking wine together there was a soft music playing in the background which showed the audience that it is a romantic and happy scene. Also, when the German war planes were overhead, the tanks rolling down the road towards Paris and the German troops crossing the river the music that was playing in the background was loud which illustrated to the audience that there is danger. When Rick was going o take the train from Paris there was the sound of heavy rain at the station, and when the conductor says All aboard, last train leaving in three minutes this demonstrates to the audience a very anxious and nervous scene to whether Ilsa will be showing up or not. After remising there was a freeze shot where no music was played and suddenly music was played when Ilsa came in this illustrated that this is when something will unexpectedly happen. Lighting Moreover, Casablanca shows many techniques of film noir, mainly with the use of shadows and lighting throughout the film. The effect of lighting in Ricks Flashback to Paris is the only bright sequences in the whole film that helps the audience to understand the happiness of Rick and Ilsa when they were in Paris. Rick is sitting, drunk and in a total dark room remembering the good times he had with Ilsa in Paris. The sudden change from dark to light illustrates to the audience the comparison of how Rick was happy in Paris and unhappy now. The final scene in Paris, where Rick is waiting under the rain for the train, the cloudy gloomy clouds and dimmed lighting on the scene indicates that Ilsa will not be showing up. It tracks the set up pattern of the light scenes are the pleased and joyful and the dark scene are sad and misery. In addition, the logo in Paris cafà ©, a shadow on a white background, this shows the whole scene a bright and happy tone. This illustrates for the audience t he mood and purpose of the flashback. Moreover, the name of the cafà © La Belle Aurore which indicates the meaning of the beautiful sunrise; this is another point of the good times they have experienced in Paris. On the other hand, the logo for Ricks Cafà © Ricks Cafà © Americian, has white letters on a dark background which shows the differences between both cafes. This use of lighting demonstrates the happiness in Paris and the sadness in Casablanca. Furthermore, in Casablanca Ricks face is glowing through the whole film; however in one scene his face was not glowing (this shows that the shadows on Ricks face demonstrates the loss of the power). This scene is when Rick was drunk waiting for his love Ilsa in his cafà ©. This point out that Rick has lost all the power and that Ilsa holds significant influence on him. According to Louis Giannetti in Understanding Movies, the use of light and shadows creates mood and emotional impacts on films. While using spotlights, which are hi ghly selective in their focus and intensity, the director can guide the audiences eyes to any place of the scene. There are various different styles of lighting, usually is designed as a lighting key, the style is connected to the theme and mood of the film, as well as its type. Tragedies and melodramas films are usually lit in high contrast, with harsh shafts of lights and dramatic streaks of blackness such as Casablanca. In general, films have darkness suggests fear, evil, and the unknown. On the other hand, lightness suggests truth, joy, and security. (Giannetti, 2010) Dialogue In addition, the dialogue of this scene is very touching to the audience. After the Cafà © has closed and streets are empty, Rick is depressed sitting in a dark room while smoking cigarettes and drinking alcohol heavily. Ricks face is completely unreadable. Sam feels that Rick is troubled and says to Rick Boss go home to bed. Rick stubbornly tells Sam that he is waiting for a lady Rick is expecting Ilsa to come back to him. Shes coming back; I know she is coming back. This shows the desperation and the bad mood Rick is feeling. Then Sam again feels that Rick is trouble and suggests that they should take the car, get drunk and drive all night or stay away until Ilsa is gone so Rick can avoid seeing Ilsa. This shows that Sam is worried about Rick. Before Rick starts to reminisce about the good memories both of them had, he then pound his fist down on the table and says out of all the cafes in town she has to walk in mine. Then Rick raises his head, trying to get his control and power back Rick madly orders Sam to repeat the song he played for Ilsa As Time Goes By: Rick: What is that your playing? Sam: Oh, just a little something on my own Rick: Well, stop it! You know what I want to hear Sam: No, I do not Rick: You played it for her; you can play it for me Sam: Well, I do not think I can remember Rick: If she can stand it, I can. Play it! While hearing As Time Goes By, the camera blurs into a dissolve from his face into a flashback- it takes Rick back to happier moments with the romance he had with Ilsa in Paris. Ricks remembers the past to oppose the explanation that Ilsa will tell Rick of her disloyalty in Paris. Rick feels rejected because of his true feeling for Ilsa and Ilsa leaving him in Paris without any explanation. At the end of Ricks remembering, the camera pans from left to right, locating Rick drunk. The camera relocates Rick on the left when unexpectedly the door of the cafà © opens. Ilsa appeared wearing a scarf and a white coat. As Rick expected, Ilsa has come back to him, but she gets up Ricks angry feelings by telling him that she would not have came to Casablanca if she had known he was here. Ilsa tries to talk to Rick but he refuses to hear the explanation she has. Rick hesitates in talking: Rick: Why did you have to come to Casablanca? There are other places. (This showed the audience how miserable he is when he found his true love with another man). Ilsa: I would not have come if I had known that you were here. Believe me, Rick, it is true. I did not know. Rick: it is funny about your voice, how it has not changed. I can still hear it. Richard dear, I will go with you any place. We will get on a train together and never stops. (Rick saying this to Ilsa shows that he is being sarcastic and he is hurt). Ilsa: Please do not. Do not Rick! I can understand how you feel. Rick: Huh! You understand how I feel. How long was it we had, honey? Ilsa: I did not count the days. Rick: Well I did. Every one of them mostly, I remember the last one the wow finish. A guy standing on a station platform, in the rain with a comical look on his face, because his insides had been kicked out. After completing the conversation, Ilsa tries to continue explaining her past story, with tears in her eyes, the reason for keeping her secret from him earlier. But Ricks rude sarcasm and irritation stops Ilsa from continuing her explanation. Editing Furthermore, editing is a very important technique to any film. Editing is used expansively and is more stressed than other film techniques such as panning or dolly shots, angels, light, mise en scene and tracking. Editing is not only important for the storyline of the film but it is significant in the sense that it allows for the engaging of several types like action, romance, and history. Through the use of cuts, the director can show numerous coincident scenes at ones that represent a different genre. According Louis Giannetti in Understanding Movies, editing is and artistic and narrative process by which scenes shot for a film are put together to create a logical visual and a narrative form. One of the most important elements about editing is that it removes the unnecessary time and space in the film. With the connection of ideas, editing associates one shot with another or one scene with another. (Giannetti, 2010) Production Design The main point of a film is that it tells the audience the story. All the films are based around a plot or storyline that includes many scenes and sequences all of which it contributes to the overall story. The use of the camera and editing is an important element of moving the image language. The director uses basic elements to get their point to audience, such as: the dialogue sequences that occur by a series of shots from one actor to another, the sequences of the scenes that will point out their point in the film. The lighting of the film can show who, where and what is important. The music can illustrate the importance of the scene. They can also point out their point to the audience by illustrating it as a story such as Casablanca the director used a storytelling technique. The director raises the some types of questions that would be asked of any manuscript document- questions about its information content, its background context, and its historical influence. Also, demands of the close reflection of camera angle, lighting, shot composition, editing and the ways in which each of these and other elements of visual language add slight patterns of understanding. If not a frame-by-frame or shot-by-shot analysis, surely a scene-by-scene or sequence-by-sequence breakdown is essential for understanding the order of images and how they may play on one another and interact with whatever soundtrack there may be. (OConnor, 1988) Sum Up To sum up, lighting, movements, sound, actors, dialogue, and editing are very essential in a film. Therefore, these elements help to provide a vivid image in the audiences head, to point out their main issue across the film, and to emphasis the important parts throughout the whole film. The director should know all of the types of elements to make sure he/she uses the correct element added in the film. If the director uses the wrong element in the film he/she may be sending a wrong message or picture to the audience. Argument of Films Reflecting the Political and Social Mores of Society at the Time of Creation Some historians argue that films reflect the political and social mores of society at the time of creation and some say otherwise. This question is arguable because it can either be that the film reflects the political and social more of the society or the society drives the entertainment. However, social and political issues can affect films and films can affect the society. Any film can be based on a political issue or a social issue that is happing in the world for example, like financial crisis there has been many films made about it or based on a history of the famous piano player Wolfgang Amadeus Mozart and Antonio Salieri in Amadeus. Films can be based on a social issue that has to do with under age prostitutes, buying illegal guns such as Taxi Driver; this kind of social issue can be seen in the world. Films can do what books cannot do: provide the audience with a story, as well as an image to back up the story. Watching a film or a commercial is a very inactive activity, allowing the audience to sit, stare and absorb everything that is presented in the screen. Films can symbolize a powerful element that can spread the ideas in a quicker form. Film-makers often manipulate their audience into believing that what they are seeing is the truth. Some film makers can also twist the truth in the film. By using a variety of techniques in their film, an audiences feeling towards one subject or another can change after watching the film. While watching the film no matter what their political or social views, their views can be changed towards the end of the movie. This kind of reaction to the audience is because of the lighting, camera movements angles and overall screen play on the film. Films can affect society in various ways. For instance, many films can affect the audiences attitudes an d morals like horror and violent films in some cases the film makes the audience act out the way they see it in the film. The audiences can start acting violent and aggressive and by doing that they will affect the society as a whole. Moreover, war films often have big bodily fit men as sergeants and most of time they would be smoking cigarettes when showed in films. Many people started buying cigarettes to be like those men this also affects the society. According to The Journal of Marketing, the president of the California state senate recently held a hearing on the issue because he believes that screen smoking does have an impact on young people and he wants the industry to respond to this problem. (Pechmann Shih, 1999). This example shows the audience that films do affect the society and the industry. Drug films also teach the audiences that doing drugs makes the audience look cool or selling drugs can make you rich. Romance films show the audience that everything ends happily when in reality not everything ends happily. Those examples changes the way the audience sees things and changes in their beliefs, perspectives and values which also affects the society. Films are cultural works of art that are formed by cultures, which reflect those cultures. Films are considered to be a vital art form, a source of popular entertainment and an influential method of educating of teaching the audience. According to the book History in Images/ Images in History: Reflections on the Importance of Film and Television Study for an Understanding of the Past, what films hides in the cinematic gift are the moral narratives needed to maintain the society that produces and supports these stories in films in the first place. Film packages this social gift in alluring visual images, economic clarification, romantic myths, political explanations, emotional validation, and convincing religious and legal value systems. The generis narratives that characterize much of the current cinema are intended to be traditional are designed to generate a sense that the audience has moral responsibility to head the warning of the film. The advice which comprises the cinematic gi ft is concerned with how to be recognized and how to function as a proper individual in the particular society that offers the film. The audience is exposed to socially excused instructions about how the society and individuals should ideally function. If the film gives the audience social misinformation understood in terms of entertaining story, the audience must, according to the laws of reciprocity give it back a promise to maintain the type of society that will continue to create and support stories. Some audiences see life as if it were just like in the movies, films that are created and controlled by a small number of audiences who maintain a limited vision of the social as well as the individual body. (Mascia-Lees Sharpe, 1992). Since 1930s, films have become major factors in politics and the culture. Film and culture implement an overly simple, reflective model of the film-culture. The issue demands some understanding of theory and recognition of the need to understand a film as one part of a much larger, complex and ever-changing culture. Understanding a film as artifact needs a consideration of its production background, the difficult joint process involved in its creation, and the political or other reasons a film may be meant to serve. Based on the acknowledgment that the audience may read in a single film differently, attention has focused on the ways in which class, gender, and political associations influence the understanding of a films signs and symbols. Film makers should draw awareness to equally vital differences in the meaning films hold for people in various cultures or other eras. Film makers should ask themselves if the films current at the time, social or culture influences that may have le aning the audiences at some specific place and time in the past to react to a film in one way or another. (OConnor, 1988). Conclusion In other words, this is a debatable issue; research could not come to an answer, whether films reflect the political and social mores of society at the time of its creation due to the fact that it depends on each societys value and priority. However, film has a huge influence on forming our thinking or it sometimes reflects real life, the issue is important. Society can drive the entertainment industry. Social and political mores of society can affect films which create a film within a specific moral, social or political, for example, Casablanca it is driven by the society with a political issue since it was set at the time of War World II and the film was about the refugees trying to escape to America.

Sunday, January 19, 2020

Hsm 541: Week 2 †You Decide

HSM 541: Week 2 – You Decide Krystal Richards Professor Alan Flower Middlefield Hospital has been a great healthcare facility to the Middlefield area. Middlefield Hospital consists of a 450-bed tertiary facility in a major urban area in the Northeast. The hospital is an integrated health system that provides the full array of inpatient and outpatient services. The hospital enjoys a reputation of quality care in the area. In recent years, there are areas of concern that need to be addressed.As I have been assigned the position as the new Chief Executive Officer (CEO) of Middlefield Hospital, I will provide strategies and recommendations needed for implementation to turnaround the hospital into a more positive experience not only for the patients but for the employees as well. My approach will implement a triad of care – access, cost, and quality. Middlefield Hospital has been a key staple within our market area of Middlefield. The hospital was able to build another facil ity on the other side of town.The new hospital offers much better facilities and the technology is state of the art. While visiting the new hospital, I noticed that the staff appeared much more invested in the quality of patient care and the patients also seem happier. As with new facilities, there comes lots of excitement. The quality of our old facility needs to be upgraded and needs some new type of organization. There needs to be a balance of power so that the new hospital is able to start performing better. This lies within the hospital medical staff, hospital administration, and hospital governance.With the hospital medical staff we need to take into account their pay for their performance on the work they do. This will in turn encourage them to want to stay with the hospital knowing they will have a steady long-term position. Hospital administration is another important thing to take into account since they work with the clients just as much as the medical staff does. From he alth insurance, to IT, to security everyone plays a key part in making sure that the patients feel comfortable. To mprove the performance, there need to be upgrades with the systems that the hospital uses to keep track of the patients information. I recommend using Epic as a tool to help keep all of the patient’s information confidential and easy to access with a click of a button. This will allow for every department to have access to a certain patient’s file whether to be from a doctor entering notes about the patient’s visit, to health insurance billing department making sure that copays and such are being paid and their accounts are kept up-to-date.The hospital governance plays an important role because of the need for funds to help keep the hospital. By providing more outside events to raise money for the hospital will be a great start to being able to fully provide for each patient that comes through Middlefield Hospital doors. Since the employee morale is going down, I think it is important to obtain a good relationship with the local university, Essex University, to provide jobs and internships to the students in the nursing program.This nursing program will make sure that our managers will be able to teach classes at the university and help train the students to be prepared to work in the hospital. This should address the problem of the employee turnover rate to decrease and bring down the number of nursing vacancies. Nurses are a very important part of the hospital because they are by the patient’s side more than the doctor can be, so they have to show compassion all of the time. This is not a hard characteristic for them to have because they already know how much helpful being a nurse can be.Also by providing better compensation packages to prospective employees might help as well. Middlefield Hospital needs the best of the best in terms of medical staff. Now that there is another hospital in the area, we need to make sur e that both hospitals can be top notch at all times. As with all plans and strategies, this is just the beginning. I believe that Middlefield Hospital will be on top in our marketing areas. By planting these seeds, I am sure that our hospital will continue to grow and become a valuable part of the community.

Saturday, January 11, 2020

Light-Induced Transformation of Amyloplasts Into Chloroplasts

Plant Physiol. (1984) 75, 142-145 0032-0889/84/75/0 142/04/$01. 00/0 Light-Induced Transformation of Amyloplasts into Chloroplasts in Potato Tubers' Received for publication November 9, 1983 and in revised form January 19, 1984 YU S. ZHU, DENISE L. MERKLE-LEHMAN, SHAIN D. KUNG* Department of Biological Sciences, University of Maryland Baltimore County, Catonsville, Maryland 21228 ABSTRACT The transformation of amyloplast into chloroplasts in potato (Solawum tuberosum L. ) tuber tissue can be induced by light.Excised potato tuber discs illuminated with white light of 3000 lux bepgn to synthesize chlorophyll after a lag period of 1 day, and continued to synthesize chlorophyll for 3 weeks. In this paper we present evidence, based on ultracentrifugal sedimentation and immunoprecipitation, that the lightmediated synthesis of Ribulose-1,5-bisphosphate carboxylase began 1 day after illumination with white light. When illuminated the chloroplasts isolated from light-grown potato tuber tissue incorporated I3SImethionine into polypeptides, one of which has been idenified as the large subunit of Ribulose-1,5-bisphosphate carboxylase.These chloroplasts are functional as determined by 02 evolution in the Hill reaction. A great deal of data is now available on the biochemistry of the development of chloroplasts from etioplasts (6). Much less is known about the development of chloroplasts from amyloplasts, a starch storing organeile, which exists in storage tissue, roots, and some callus. It is well known that potatoes turn green on exposure to light for several days.Not only does the study of the organelle transformation and light-regulated gene expression stimulate theoretical interest, but the greening of potato also has some practical importance, since the greening of the potato is accompanied by the formation of the poisonous alkaloid, solanine (5). The morphological and ultrastructural changes in the development of chloroplasts from amyloplasts were described in several electron microscopic studies (3, 11). In this communication we present some biochemical evidence to show the function of chloroplasts from potato tuber based on the biosynthesis of Chl, RuBPCase,2 and Hill activity. lates in sterile Petri dishes (9 cm), 40 discs per dish. The discs were illuminated at room temperature by fluorescent tubes with intensity of 3000 lux. Chlorophyll and RuBPCase Determination. Chl was determined according to Arnon (2). RuBPCase was detected with a Model E analytical ultracentrifuge, as previously described (9). Schlieren pictures were taken at 44,770 rpm, 10 min after attaining this speed. An Ouchterlony double diffusion test was employed to determine the RuBPCase specifically. The antiserum was prepared against RuBPCase from tobacco (15).Light-Driven Protein Synthesis in Choroplasts. Ten g of green potato tuber discs which had been exposed to white light for 7 d were homogenized in a Waring Blendor with cold isolation buffer (sucrose 0. 35 M, Hepes-NaC l 25 mm, EDTA 2 mM, isoascorbateNa 2 mm, pH 7. 6) and filtered through 2 layers of Miracloth. The resultant filtrate was centrifuged at 30g for 1 min to remove starch granules, and the supernatant was then centrifuged at 2500g for 1 min. The pellet was resuspended in 1 ml of KCI suspension (KCI 0. 2 M, Tricine-KOH 66 mm, MgCl2 6. 6 mM).The chloroplast suspension was transferred to a Petri dish (3 cm in diameter) and incubated with [135S]methionine (10 ,uCi) at room temperature under white light (4000 lux). During a 2 h incubation, the incorporation of [35S]methionine into proteins was measured. After the 2 h incubation the radioactive polypeptides were separated on an SDS-polyacrylamide gradient gel (815%), followed by fluorography as described by Blair and Ellis (4). 02 Evolution Assay. The ability of chloroplasts from potato tuber discs to evolve 02 was measured in the Hill reaction ccording to the procedure of Marsho et al. (12). The incubation system consisted of ferricyanide 1. 7 mm, methylamine 3 mm, glyceraldehyde 10 mm, and chloroplasts corresponding to 15 yg of Chl in a total volume of 0. 6 ml. The reaction was initiated by irradiation with red light (22. 4 mw/cm2). The 02 evolution was measured and recorded with a polarograph. RESULTS MATERIAILS AND METHODS Chlorophyll Synthesis in Light-Illuminated Potato Tuber. Our Preparation, Culture, and Light Treatment of Potato Tuber experiments showed that the greening of potato tuber depended Discs.Centennial and Katahdin, two varieties of potato tubers on varieties, storage temperature, light intensity, and wavelength supplied by the United States Department of Agriculture were of light. Out of more than ten varieties of potatoes tested, two used in this study. The tubers were peeled and sterilized with varieties, Centennial and Katahdin, which turn green more Amphyl (National Laboratories, Lehn and Fink Industrial Prod- quickly under light, were selected in this study. Storage of potato ucts Division of St erling Drug Inc. , New Jersey).Discs (10 x 2 tuber below 4 °C retarded or inhibited the transformation of mm) were made with a sterile cork borer and a gel slicer. These amyloplasts into chloroplasts. Blue light was most effective in discs, after washing in sterile water, were placed on 1% agar inducing greening of potato tubers. No red light stimulation was observed. Potato tuber discs illuminated with white light began to green ‘ Supported by National Institutes of Health grant CM22746-01 and with a lag period of about 1 d. The greening continued for 3 United States Department of Agriculture agreement 58-32044157. 2Abbreviations: RuBPCase, ribulose-1,5-bisphosphate carboxylase; weeks after culture (Fig. 1). After illumination for 3 weeks, the potato tuber tissue contained 10 Ag Chl/g of fresh tissue, about LS, large subunit. 142 TRANSFORMATION OF AMYLOPLASTS IN POTATO TUBERS 143 14 oct[L] 15 – a) 121 10 101 5 C-) 8 x 0 > ct [D] Days After Illumination FIG. 1. Time c ourse of the synthesis of chlorophyll in potato tuber discs during continuous illumination with white light. lAt [D] By 0 15 I †¦.. AAt [L] i _x- 60 120 90 Time (min) FIG. 3. Incorporation of [35S]methionine into proteins in the lightdriven chloroplast protein synthesis system. -M1 samples were added to 20% TCA containing 10 tg/ml unlabeled methionine. Proteins were precipitated with 5% TCA containing 10 Mg/ml unlabeled methionine, and counted with a Mark I scintillation counter. Ct, chloroplasts; At, amyloplasts (control); L, light-incubated; D, dark-incubated. FIG. 2. Biosynthesis of RuBPCase in potato tuber tissue as assayed by Ouchterlony double diffusion 0 h (1), 6 h (2), 12 h (3), 24 h (4), 48 h (5), and 72 h (6) after illumination. The central well contained antibody to LS. One g of potato tuber discs was homogenized with 0. 1 ml of Tris 80 mm, MgCl2 20 mm, KCI 40 mM, pH 8. . After centrifugation at 12,000g for 10 min, 20 Ml of the supernatant were used for assay of RuBP Case. hundredth of the Chl content in normal leaves. This is primarily due to fewer chloroplasts per cell. The chloroplasts were not distributed uniformly in discs and were probably linked to some specific ultrastructure in the cortex. Microscopic observation revealed that the amyloplasts were comprised of two types: large (55 x 80 Mm) and small (5-20 Mum). Most of them were stainable with I2-KI. Upon exposure of the discs to light the small amyloplasts only turned greenish.RuBPCase Biosynthesis in Potato Tuber during Greening. Since RuBPCase is the most abundant soluble protein in the chloroplasts (10), it can be used as an important biochemical marker of chloroplasts. The de novo synthesis of RuBPCase was clearly demonstrated by ultracentrifugal sedimentation (data not shown) and specific immunoprecipitation (Fig. 2). The Schlieren pattern of extracts from normal leaves consists of four peaks representing 80S cytoplasmic ribosomes, 70S chloroplast ribosomes, 18S Fraction I protein (RuBPCase), and 4 to 6S Fraction II proteins (8, 9).The extract from potato tuber stored in the dark lacked the peak of RuBPCase, whereas a small peak was observed in discs exposed to 3 d of light, indicating the light one initiated the synthesis of RuBPCase (data not shown). The biosynthesis of RuBPCase was also demonstrated by an Ouchterlony double diffusion assay (Fig. 2). The light-mediated synthesis of RuBPCase started at 1 d after illumination and increased during greening. The RuBPCase content was estimated to be -6 ,ug/g fresh potato tuber tissue, whereas the RuBPCase content of a typical green leaf is 5 to 10 mg/g fresh tissue (10).Further evidence for the de novo synthesis of RuBPCase in light-treated potato tuber was obtained from the experiment on light-driven protein synthesis in chloroplasts. The chloroplasts, isolated from potato tuber discs after illumination for 7 d, exhibited a higher activity of protein synthesis, as demonstrated by the incorporation of [35S]meth ionine into proteins (Fig. 3). In contrast to light-driven protein synthesis in chloroplasts, the chloroplasts in the dark and especially amyloplasts, either in the light or dark, exhibited a very low protein synthesis activity.A number of radioactive polypeptides synthesized in the chloroplasts in the light were recognized on SDS-polyacrylamide gels followed by fluorography (Fig. 4). It was observed that some chloroplast polypeptides (mol wt 39,000, 50,000, 52,000, 55,000, 94,000, 96,000) were synthesized more readily in the light than in the dark. One of these polypeptides co-migrated with purified unlabeled LS of RuBPCase (mol wt 52,000), and was identified as the LS, based on this and results presented in Figure 2.There is also one strongly light-initiated polypeptide (mol wt 64,000) which does not appear in the dark. 02 Evolution of Chloroplasts from Light-Induced Potato Tubers. Upon illumination with red light, the chloroplasts, isolated from light-treated potato tuber discs a fter 3 weeks of light treat- 144 Plant Physiol. Vol. 75, 1984 ZHU ET AL. sure to light, the amyloplasts are transformed into chloroplasts, which have a different function. The changes in ultrastructure of potato tuber amyloplasts during greening were investigated by electron microscope.It was shown that the main developmental features were elongation of vesicles into thylakoids, the differentiation of grana and the appearance of ribosomes in the stroma (3), although the ultrastructure is generally less well developed compared to that for normal leaves. On the other hand, there is very little information on biochemical alterations in potato tuber during greening. To confirm the transformation of amyloplasts into chloroplasts, this study provides some biochemical evidence: (a) the synthesis of photosynthetic pigments, (b) the synthesis of RuBPCase and other proteins, and (c) Hill reaction activity.Before illumination the potato tubers contain no Chl or Pchl, but do contain carotenoid which increases during greening (1). Very low light intensity (400 lux) was required to initiate greening. The light may penetrate into the potato tuber discs and evoke the development of chloroplasts from amyloplasts. During the development of chloroplasts from amyloplasts, assembly of Chl into the newly synthesized membranes occurs. Cold storage may cause the breakage of membranes (13); however, some studies suggested that amyloplast membranes remain intact in cold storage (14).It was frequently observed that the chloroplasts were formed as streaks in the potato tuber discs, suggesting a special structure is linked to the chloroplast development. The Schlieren pattern of the extracts from light-induced potato tuber tissue as well as its immunoprecipitation reaction with antiserum to RuBPCase demonstrated that RuBPCase, an important enzyme in the photosynthetic carbon cycle, was de novo synthesized during greening. This result was further confirmed by the active light-dependent pro tein synthesis in the isolated chloroplasts.The biosynthesis of RuBPCase during greening suggests the involvement and operation of CO2 assimilation in the chloroplasts. Furthermore, the photosynthetic function of electron transport and 02 evolution in isolated chloroplasts from potato tuber was shown by the high Hill reaction activity. The transformation of amyloplasts into chloroplasts is absolutely light-dependent. The fact that red light did not stimulate this transformation indicates that phytochrome may not participate in this regulation. This coincides with the result that potato tuber does not have phytochrome (7).What is the photoreceptor in this light-induced organelle transformation? How does light turn on the genes for the development of chloroplasts, and turn off the genes for the development of amyloplasts? Undoubtedly, this system provides an attractive and challenging model for investigations into the molecular mechanisms underlying the photoregulation of development and gene expression. FIG. 4. SDS-PAGE of light- (1) and dark- (d) initiated polypeptides synthesized in chloroplasts isolated from light-treated potato tuber discs. (R) photograph of stained gel. L) fluorograph of (R). Arrows indicate the mol wt of light-stimulated polypeptides and band corresponding to LS. Although the dark-incubated sample was more concentrated than the light-incubated sample (R), the fluorograph (L) indicates there was more incorporation of [35S]methionine into the light-induced polypeptides than into the dark-initiated polypeptides, demonstrating that more polypeptides were synthesized in the light than in the dark. The samples were incubated with [35S]methionine for 120 min. ment, evolved 02 using ferricyanide as an electron acceptor.This Hill reaction activity is comparably high (177 Amol 02/mg Chl h), indicating that an active electron transport reaction took place in this chloroplast preparation. DISCUSSION The cells of potato tuber contain a large number of amyloplasts, whose function is to accumulate and store starch in the form of reserve starch granules. It is of interest that upon expo- Acknowledgments-We thank Dr. Ray Webb of United States Department of Agriculture, Beltsville, Maryland for providing us with different varieties of potato, and F. J. Xi for her help in determination of Hill activity. LITERATURE CITEDDevelopment of chloroplasts from amyloplasts in potato tuber discs. New Phytol 72: 449-463 2. ARNON DI 1949 Copper enzymes in isolated chloroplasts. Polyphenoloxidase 1. ANSTIS PJP, DH NORTHCOTE 1973 in Beta vulgaris. Plant Physiol 24: 1-15 3. BADENHUIZEN NP, R SALEMA 1976 Observations of the development of chloroamyloplasts. Rev Biol (Lisb) 6: 139-155 4. BLAIR GE, RJ ELLIS 1973 Protein synthesis in chloroplasts 1. Light-driven synthesis of the large subunit of fraction I protein by isolated pea chloroplasts. Biochim Biophys Acta 319: 223-234 5. FORSYTH AA 1954 British Poisonous Plants.Her Majesty's Stationery office, L ondon 6. KIRK JTO, RAE TILNEY-BASSErr 1978 The plastids. Growth and Differentiation of Plastids. Part I. Formation of the Chloroplast during Greening of the Enolated Plant. Elsevier/North-Holland Biomedical Press, Amsterdam, The Netherlands, pp 720-773 7. KOUKKARI WL, WS HILLMAN 1966 Phytochrome levels assayed by in vivo spectrophotometry in modified underground stems and storage roots. Physiol Plant 19: 1073-1078 TRANSFORMATION OF AMYLOPLASTS IN POTATO TUBERS 8. KUNG SD 1977 Expression of chloroplast genomes in higher plants.Annu Rev Plant Physiol 28: 401-437 9. KUNG SD, PR RHODES 1981 Hormonal effects on the biosynthesis of tobacco RuBPCase in vitro. Beitr Tabakforsch Int I 1: 44-49 10. KUNG SD, TC Tso 1978 Tobacco as a potential food source and smoke material: soluble protein content, extraction, and amino acid composition. J Food Sci 43: 1844-1852 11. LoBov UP, PI BONDAR 1977 The RNA of potato tuber amyloplasts. Fiziol Rast 24: 318-322 12. MARSHO TV, PM SOKOLOVE, RB MACKAY 1980 Regulation of photosynthetic 145 electron transport in intact spinach chloloroplasts. Plant Physiol 65: 703-706 13.OHAD I, I FREIDBERG, Z NEEMAN, M S(CHRAMM 1971 Biogenesis and degradation of starch 1. The fate of the amyl,loplast membrane during maturation and storage of potato tubers. Plant Phyysiol 47: 465-477 14. WETZSTEIN HY, C STERLING 1978 Integri' ity of amyloplast membranes in stored potato tubers. Z Pflanzenphysiol Bd 90DS: 373-378 15. ZHU YS, PS LovErr, DM WILLIAMS, SID KUNG 1983 Nicotiana chloroplast genome 7 expression in E. coli and BB. subtilis of tobacco and Chlamydomonas chloroplast DNA sequences cooding for the large subunit of RuBP carboxylase. Theor Appl Genet 67: 3333-336

Friday, January 3, 2020

Schizophrenia And Its Effects On Society - 1739 Words

Abstract Schizophrenia is one of the largest most delirious disorders that affect individuals all over the world. An individual diagnosed with schizophrenia loses touch of reality, confused by the world within their head and the outside world a.k.a. reality. If left untreated schizophrenia could have a largely negative impact on the individual and others around them. Because of this it is highly important that schizophrenics get the treatment they need, such as antipsychotic drugs, to get back to their normal, healthier lives. Introduction According to Ken Duckworth of NAMI (2013), one in four Americans suffer from mental illness. Of these mental illnesses, schizophrenia is one of the most delirious disorders that affects the largest majority of the populations. According to textbook writer Ronald Comer (2012-2013), 1 in 100 people within the world suffer from this disorder, 24 million worldwide, 2.5 million being in the U.S... The staggering fact is that those statistics are only of the diagnosed population. Meaning there are others still out there that haven’t been diagnosed or treated suffer the disorder. For individuals of schizophrenia there are different side effects, types, treatments, and many dangers of schizophrenia if left untreated. A person with schizophrenia is unaware of the disorder, because this disorder blurs the reality. The disorder becomes their reality and can take over lives, or take lives. It is important for the public to beShow MoreRelatedSchizophrenia And Its Effects On Socie ty1819 Words   |  8 PagesSchizophrenia is a severe brain disorder in which it is hard to distinguish the difference between what is real and what is not. Schizophrenia has been found throughout recorded history but was not considered a worthy medical condition until the eighteenth century. It is also considered today a leading public health problem that has enormous personal and economic costs worldwide. Schizophrenia involves a disturbance of thought, perception, emotion, movement, and behavior. The symptoms vary acrossRead MoreSchizophrenia And Its Effects On Society1195 Words   |  5 PagesSchizophrenia is a considered a severely chronic, disabling brain disease. Sufferers that have schizophrenia tend to experience hallucinations, delusions and sometimes distorted reality. When an individual is diagnosed with schizophrenia, they are physically inept to acting out â€Å"everyday functions†. With careful research, it is now known nearly one percent of the American popu lation develops schizophrenia. Unfortunately, that portion is about two million american citizens and the numbers are slowlyRead MoreSchizophrenia And Its Effects On Society1464 Words   |  6 PagesAccording to World Health Organization schizophrenia is listed as the second highest contributor to overall burden of diseases. Schizophrenia is one of the most tragic diseases a human being can be afflicted with. 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